Large-scale Purification and Refolding of Recombinant Eel Growth Hormone
نویسندگان
چکیده
منابع مشابه
Large-scale purification and refolding of recombinant eel growth hormone.
In the past decade, growth hormones (GHs) have been isolated from the pituitaries of several teleost species including tilapia, salmon, carp, and eel.1~9) Although teleost GHs may be useful in promoting growth in aquaculture, little is known about appropriate routes, doses, or patterns of administration. Considerable amounts of teleost GHsare required to clarify both the mode of action of teleo...
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Production and purification of human growth hormone using a simple method was studied in two recombinantEscherichia coli, D7-5 and C27-2 strains. The r-hGH was expressed in the form of inclusion body in a batchfermentation process and purified to 99% purity using a procedure based on acid precipitation of the hostderived proteins and other impurities. The effect of the pH and ...
متن کاملPersian sturgeon growth hormone elaboration and purification
In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...
متن کاملPersian sturgeon growth hormone elaboration and purification
In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 &muL isopropyl &beta-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours an...
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Background: Interferon α-2b is a vital biotherapeutic produced through the recombinant DNA technology in E. coli. The recombinant IFN-α2b normally appears as intercellular IBs, which requires intensive refolding and purification steps. Method: Purification of IFN-α2b from solubilized IB was performed using two-phase extraction. To optimize refolding conditions, the effects of pH and different a...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1991
ISSN: 0002-1369
DOI: 10.1080/00021369.1991.10870807